5-methylcytosine Monoklonaler Antikörper

5-methylcytosine Monoklonal Antikörper für IHC, Dot Blot, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

m5C und mehr (2)

Anwendung

WB, IHC, IF, RIP, Dot Blot, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

CloneNo.

2C9G9

Kat-Nr. : 68301-1-Ig

Synonyme

m5C, 5mC



Geprüfte Anwendungen

Erfolgreiche Detektion in IHCMaushodengewebe, Rattenhirngewebe, Rattenhodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in ELISAcompound
Erfolgreiche Detektion in Dot BlotHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:2500-1:10000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
DOT BLOTDOT BLOT : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

68301-1-Ig bindet in WB, IHC, IF, RIP, Dot Blot, ELISA 5-methylcytosine und zeigt Reaktivität mit m5C

Getestete Reaktivität m5C
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Verbindung
Vollständiger Name 5-methylcytosine
Gene symbol
Gene ID (NCBI)
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216). This antibody can recognize both 5mC in DNA and m5C in RNA.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68301-1-IgDotBlot.pdf

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IHC protocol for 5-methylcytosine antibody 68301-1-IgProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human

Cancer Lett

NSUN4 mediated RNA 5-methylcytosine promotes the malignant progression of glioma through improving the CDC42 mRNA stabilization

Authors - Zhen Zhao
human

Biomedicines

NSUN2 Promotes Head and Neck Squamous Cell Carcinoma Progression by Targeting EMT-Related Gene LAMC2 in an m5C-YBX1-Dependent Manner

Authors - Shuojin Huang
humanWB

Redox Biol

NSUN2 lactylation drives cancer cell resistance to ferroptosis through enhancing GCLC-dependent glutathione synthesis

Authors - Kaifeng Niu

Cancer Immunol Immunother

NSUN2/ALYREF axis-driven m5C methylation enhances PD-L1 expression and facilitates immune evasion in non-small-cell lung cancer

Authors - Yiran Yang
humanIHC

J Exp Clin Cancer Res

NONO regulates m5C modification and alternative splicing of PTEN mRNAs to drive gastric cancer progression

Authors - Gaichao Zhao

NPJ Precis Oncol

piRNA28846 has the potential to be a novel RNA nucleic acid drug for ovarian cancer

Authors - Hong-Lei Qin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Lisa (Verified Customer) (08-20-2025)

I used this Antibody for slot-blotted RNA and it worked well with low background.

  • Applications: Western Blot, Other
  • Primary Antibody Dilution: 1:5000
FH

DONGHONG (Verified Customer) (07-02-2024)

I used this antibody for the competitive elisa kit. Coating BSA-m5C and blocking the 96-well plate. Use the the standard to competitive bind m5C antibody. However, the standard curve was not good as m5C from Abcam (ab10805).

  • Applications: Other
  • Primary Antibody Dilution: 1:1000