5-methylcytosine Monoklonaler Antikörper
5-methylcytosine Monoklonal Antikörper für IHC, Dot Blot, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
m5C und mehr (2)
Anwendung
WB, IHC, IF, RIP, Dot Blot, ELISA
Konjugation
Unkonjugiert
CloneNo.
2C9G9
Kat-Nr. : 68301-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in IHC | Maushodengewebe, Rattenhirngewebe, Rattenhodengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in ELISA | compound |
Erfolgreiche Detektion in Dot Blot | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Immunhistochemie (IHC) | IHC : 1:2500-1:10000 |
Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
DOT BLOT | DOT BLOT : 1:2500-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 1 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
RIP | See 1 publications below |
Produktinformation
68301-1-Ig bindet in WB, IHC, IF, RIP, Dot Blot, ELISA 5-methylcytosine und zeigt Reaktivität mit m5C
Getestete Reaktivität | m5C |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Verbindung |
Vollständiger Name | 5-methylcytosine |
Gene symbol | |
Gene ID (NCBI) | |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216).
This antibody can recognize both 5mC in DNA and m5C in RNA.
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68301-1-IgDotBlot.pdf
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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IHC protocol for 5-methylcytosine antibody 68301-1-Ig | Protokoll herunterladenl |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cancer Lett NSUN4 mediated RNA 5-methylcytosine promotes the malignant progression of glioma through improving the CDC42 mRNA stabilization | ||
Biomedicines NSUN2 Promotes Head and Neck Squamous Cell Carcinoma Progression by Targeting EMT-Related Gene LAMC2 in an m5C-YBX1-Dependent Manner | ||
Redox Biol NSUN2 lactylation drives cancer cell resistance to ferroptosis through enhancing GCLC-dependent glutathione synthesis | ||
Cancer Immunol Immunother NSUN2/ALYREF axis-driven m5C methylation enhances PD-L1 expression and facilitates immune evasion in non-small-cell lung cancer | ||
J Exp Clin Cancer Res NONO regulates m5C modification and alternative splicing of PTEN mRNAs to drive gastric cancer progression | ||
NPJ Precis Oncol piRNA28846 has the potential to be a novel RNA nucleic acid drug for ovarian cancer |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lisa (Verified Customer) (08-20-2025) | I used this Antibody for slot-blotted RNA and it worked well with low background.
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FH DONGHONG (Verified Customer) (07-02-2024) | I used this antibody for the competitive elisa kit. Coating BSA-m5C and blocking the 96-well plate. Use the the standard to competitive bind m5C antibody. However, the standard curve was not good as m5C from Abcam (ab10805).
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