5-methylcytosine Monoklonaler Antikörper
5-methylcytosine Monoklonal Antikörper für IHC, Dot Blot, ELISA, Indirect ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
m5C
Anwendung
IHC, Dot Blot, ELISA, Indirect ELISA
Konjugation
Unkonjugiert
CloneNo.
2C9G9
Kat-Nr. : 68301-1-PBS
Synonyme
Geprüfte Anwendungen
Produktinformation
68301-1-PBS bindet in IHC, Dot Blot, ELISA, Indirect ELISA 5-methylcytosine und zeigt Reaktivität mit m5C
Getestete Reaktivität | m5C |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Verbindung |
Vollständiger Name | 5-methylcytosine |
Gene symbol | |
Gene ID (NCBI) | |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS only |
Lagerungsbedingungen | Store at -80°C. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216).
This antibody can recognize both 5mC in DNA and m5C in RNA.
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68301-1-IgDotBlot.pdf