mCherry Polyklonaler Antikörper

mCherry Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26765-1-PBS

Synonyme

Red fluorescence protein, red fluorescent protein, RFP, RFP-tag

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  WB analysis using 26765-1-AP (same clone as 26765-1-PBS)

  mCherry tagged protein was subjected to SDS PAGE followed by western blot with 26765-1-AP (mCherry antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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  WB analysis of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

  WB result of mCherry antibody (26765-1-AP; 1:2000; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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  WB analysis of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

  WB result of mCherry antibody (26765-1-AP; 1:300; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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  IP experiment of Transfected HEK-293T using 26765-1-AP (same clone as 26765-1-PBS)

  IP result of anti-mCherry (IP:26765-1-AP, 4ug; Detection:26765-1-AP 1:2000) with Transfected HEK-293T cells lysate 1280 ug. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (26765-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26765-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA mCherry und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: mCherry fusion protein Ag25320
• Vollständiger Name: mCherry
• Berechnetes Molekulargewicht: 27 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.

The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm).

When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody raised against mCherry. It can be used to detect mCherry, dsRed, tdTomato, and mScarlet.