mCherry Rekombinanter Antikörper

mCherry Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241830F8

Rezensionen (1)

Kat-Nr. : 81202-2-RR

Synonyme

241830F8

---

• 

  WB analysis using 81202-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81202-2-RR (mCherry antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Transfected HEK-293 using 81202-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using mCherry antibody (81202-2-RR, Clone: 241830F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of Transfected HEK-293T using 81202-2-RR

  1x10^6 HEK-293T cells or mCherry Transfected HEK-293T cells were intracellularly stained with 0.25 ug mCherry Recombinant antibody (81202-2-RR, Clone:241830F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 81202-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81202-2-RR against mCherry were performed. The affinity constant is 0.205 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Transfected HEK-293T cells
• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81202-2-RR bindet in WB, IF/ICC, FC (Intra), ELISA mCherry und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: mCherry fusion protein Ag25320
• Vollständiger Name: mCherry
• Berechnetes Molekulargewicht: 27 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Red fluorescent proteins (RFPs) is a collective term referring to a heterogeneous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitating visualization of subcellular localization through fluorescence microscopy. This antibody is a rabbit polyclonal antibody raised against mCherry.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Christine (Verified Customer) (09-23-2025)

Excellent, strong and very clean antibody recognising mCHERRY tagged proteins overexpressed in HEK293T cells. No background at all.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: Transfected HEK293T