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mDia1 Rekombinanter Antikörper

mDia1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243001A5

Kat-Nr. : 85708-2-PBS

Synonyme

DIAPH1, DIAP1, DIAPH 1, Diaphanous related formin 1, Diaphanous-related formin-1

Filter:
  • Western Blot (WB) analysis of various lysates using mDia1 Recombinant antibody (85708-2-RR)

    WB analysis using 85708-2-RR (same clone as 85708-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85708-2-RR (mDia1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85708-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using mDia1 Recombinant antibody (85708-2-RR)

    IF Staining of HeLa using 85708-2-RR (same clone as 85708-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using mDia1 antibody (85708-2-RR, Clone: 243001A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85708-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85708-2-RR

    Affinity and Kinetic Characterization of 85708-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85708-2-RR against Human mDia1 were performed. The affinity constant is 3.88 nM.

Geprüfte Anwendungen

Produktinformation

85708-2-PBS bindet in WB, IF/ICC, Indirect ELISA mDia1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen mDia1 fusion protein Ag14523
Vollständiger Name diaphanous homolog 1 (Drosophila)
Berechnetes Molekulargewicht 1272 aa, 141 kDa
Beobachtetes Molekulargewicht 140-150 kDa
GenBank-ZugangsnummerBC007411
Gene symbol mDia1
Gene ID (NCBI) 1729
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).