mDia1 Rekombinanter Antikörper

mDia1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

243001A5

Kat-Nr. : 85708-2-RR

Synonyme

DIAPH1, DIAP1, DIAPH 1, Diaphanous related formin 1, Diaphanous-related formin-1

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  WB analysis using 85708-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85708-2-RR (mDia1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85708-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using mDia1 antibody (85708-2-RR, Clone: 243001A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85708-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85708-2-RR against Human mDia1 were performed. The affinity constant is 3.88 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MDA-MB-231-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85708-2-RR bindet in WB, IF/ICC, ELISA mDia1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: mDia1 fusion protein Ag14523
• Vollständiger Name: diaphanous homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 1272 aa, 141 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC007411
• Gene symbol: mDia1
• Gene ID (NCBI): 1729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).