mNeonGreen Polyklonaler Antikörper

mNeonGreen Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 29523-1-AP

Synonyme

mNeon Green, blFP-Y3

---

• 

  WB analysis using 29523-1-AP

  mNeonGreen tagged proteins and Native HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 29523-1-AP (mNeonGreen antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

29523-1-AP bindet in WB, IF, ELISA mNeonGreen und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: mNeonGreen fusion protein Ag31617
• Vollständiger Name: mNeonGreen
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: PF01353
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity. This antibody can be used to detect the mNeonGreen tagged recombinant protein.

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Unlicensed origin DNA melting by MCV and SV40 polyomavirus LT proteins is independent of ATP-dependent helicase activity; Authors - Li Wan
	WB	Elife; A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria; Authors - Sofiene Seef
human	WB,IF	Cancer Cell Int; Regulation of microtubule nucleation in glioblastoma cells by ARF GTPase-activating proteins GIT1 and GIT2 and protein kinase C; Authors - Vadym Sulimenko

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Amy (Verified Customer) (03-18-2024)

Not very clean, some non-specific bands, mNG-tagged protein can be observed. HEK293T lysates 25ug.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T