nNOS Polyklonaler Antikörper
nNOS Polyklonal Antikörper für IHC, IF-P, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
IHC, IF-P, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18984-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in IHC | Mausherzgewebe, Maushirngewebe, Mausnierengewebe, Rattenhodengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF-P | Maushirngewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| IHC | See 3 publications below |
Produktinformation
18984-1-AP bindet in IHC, IF-P, ELISA nNOS und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Hund, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | nitric oxide synthase 1 (neuronal) |
| Berechnetes Molekulargewicht | 161 kDa |
| GenBank-Zugangsnummer | NM_000620 |
| Gene symbol | nNOS |
| Gene ID (NCBI) | 4842 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1). The antibody can recognize isoform 1,2,4 of nNOS.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| IHC protocol for nNOS antibody 18984-1-AP | Protokoll herunterladenl |
| IF protocol for nNOS antibody 18984-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Mod Pathol Neuronal distribution in colorectal cancer: associations with clinicopathological parameters and survival | ||
ACS Chem Neurosci Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus. | ||
Exp Eye Res Altered transsulfuration pathway enzymes and redox homeostasis in inherited retinal degenerative diseases. | ||
Acta Pharmacol Sin Neuroprotective effects of scutellarin on rat neuronal damage induced by cerebral ischemia/reperfusion. | ||
Biochim Biophys Acta Mol Cell Res NMDA receptor activation induces damage of alveolar type II cells and lung fibrogenesis through ferroptosis | ||
Eur J Pharmacol Suppressive effect of nitric oxide synthase (NOS) inhibitor L-NMMA acetate on choroidal fibrosis in experimental myopic guinea pigs through the nitric oxide signaling pathway |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Reyes (Verified Customer) (03-01-2024) | Used nNOS antibody on human cortex brain sections (in red), it worked very nicely
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