Nectin-4/PVRL4 Polyklonaler Antikörper

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• KD/KO Validated

Nectin-4/PVRL4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 21903-1-AP

Synonyme

Nectin-4, PVRL4, Ig superfamily receptor LNIR, LNIR, nectin 4

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  WB analysis using 21903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 21903-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21903-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21903-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 21903-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (21903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, humanes Plazenta-Gewebe, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

21903-1-AP bindet in WB, IHC, IF/ICC, ELISA Nectin-4/PVRL4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nectin-4/PVRL4 fusion protein Ag14127
• Vollständiger Name: poliovirus receptor-related 4
• Berechnetes Molekulargewicht: 510 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55-80 kDa, 43 kDa,
• GenBank-Zugangsnummer: BC010423
• Gene symbol: Nectin-4
• Gene ID (NCBI): 81607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a glycosylated form of 60-80 kDa and a deglycosylated form of 55-70 kDa. The 43 kDa band immunoprecipitated in the supernatant corresponds to the soluble Nectin-4 form. (PMID: 15784625).

Publikationen

Species	Application	Title
human	WB	Mol Oncol; MiR-31 and miR-128 regulates poliovirus receptor-related 4 mediated measles virus infectivity in tumors.; Authors - Hirosha Geekiyanage
mouse	WB,IHC	Int J Urol; Chemotherapy with gemcitabine and cisplatin downregulates tumor expression level of nectin-4 in a syngeneic model of murine MBT2 urothelial cancer cell line and C3H mice; Authors - Makito Miyake
human	IHC	Oncol Lett; Upregulation of nectin-4 is associated with ITGB1 and vasculogenic mimicry and may serve as a predictor of poor prognosis in colorectal cancer.; Authors - Jinxiu Zhang
mouse,human	WB	Cell Biosci; Interferon-stimulated gene PVRL4 broadly suppresses viral entry by inhibiting viral-cellular membrane fusion; Authors - Qiaomei Cai ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgan (Verified Customer) (08-09-2021)

I had a lot of trouble using this PVRL4 antibody on western blots of 2 ovarian cancer cell lines; OV8 and OV90. I kept getting prominent bands at 250 kda. I tried cutting the 250 band to see if that would help, but it did not make the signal any better in the band(s) below. Any insight on this would be greatly appreciated. Thank you.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: OV8 and 0V90

Nectin-4/PVRL4 Antibody Western Blot validation (1:1000 dilution) in OV8 and 0V90 (Cat no:21903-1-AP)