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Nectin-4/PVRL4 Rekombinanter Antikörper

Nectin-4/PVRL4 Rekombinant Antikörper für IF/ICC, FC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240009F2

Kat-Nr. : 83223-4-PBS

Synonyme

Nectin-4, PVRL4, 240009F2, Ig superfamily receptor LNIR, LNIR

Filter:
  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Nectin-4/PVRL4 Recombinant antibody (83223-4-RR)

    IF Staining of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (83223-4-RR, Clone: 240009F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83223-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using Nectin-4/PVRL4 Recombinant antibody (83223-4-RR)

    FC experiment of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

    1x10^6 MCF-7 cells were surface stained with 0.25 ug Nectin-4/PVRL4 Recombinant Antibody (83223-4-RR, Clone: 240009F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83223-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83223-4-RR

    Affinity and Kinetic Characterization of 83223-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83223-4-RR against Human Nectin-4/PVRL4 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

83223-4-PBS bindet in IF/ICC, FC, Indirect ELISA Nectin-4/PVRL4 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Nectin-4/PVRL4 fusion protein Eg0415
Vollständiger Name poliovirus receptor-related 4
Berechnetes Molekulargewicht 510 aa, 55 kDa
GenBank-ZugangsnummerBC010423
Gene symbol Nectin-4
Gene ID (NCBI) 81607
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).