p130Cas / BCAR1 Monoklonaler Antikörper

p130Cas / BCAR1 Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D11C10

Kat-Nr. : 67215-1-PBS

Synonyme

BCAR1, 2D11C10, CASS1, CAS1, CAS

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  WB analysis using 67215-1-Ig (same clone as 67215-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 67215-1-Ig (same clone as 67215-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig, Clone:2D11C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67215-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA p130Cas / BCAR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: p130Cas / BCAR1 fusion protein Ag10604
• Vollständiger Name: breast cancer anti-estrogen resistance 1
• Berechnetes Molekulargewicht: 870 aa, 93 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC062556
• Gene symbol: BCAR1
• Gene ID (NCBI): 9564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.