DCTN1/p150 glued Polyklonaler Antikörper

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• KD/KO Validated

DCTN1/p150 glued Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55182-1-PBS

Synonyme

DCTN1, p150 glued, DP-150, DCTN 1, DAP-150

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  WB analysis of human brain using 55182-1-AP (same clone as 55182-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55182-1-AP (same clone as 55182-1-PBS)

  WB result of DCTN1/p150 glued antibody (55182-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCTN1/p150 glued transfected HeLa cells. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55182-1-AP (same clone as 55182-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55182-1-AP (same clone as 55182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 55182-1-AP (same clone as 55182-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 55182-1-AP (p150 glued antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55182-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55182-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA DCTN1/p150 glued und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dynactin 1 (p150, glued homolog, Drosophila)
• Berechnetes Molekulargewicht: 142 kDa
• Beobachtetes Molekulargewicht: 135 kDa, 150 kDa
• GenBank-Zugangsnummer: NM_004082
• Gene symbol: p150 glued
• Gene ID (NCBI): 1639
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.