Cdkn2a/p16INK4a Polyklonaler Antikörper

Cdkn2a/p16INK4a Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 28416-1-AP

Synonyme

Cdkn2a, p16INK4a, Cyclin-dependent kinase inhibitor 2A, p16, p16(INK4a)

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  WB analysis using 28416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (Cdkn2a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (p16INK4a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C6-Zellen, Maus-Uterusgewebe, Neuro-2a-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

28416-1-AP bindet in WB, ELISA Cdkn2a/p16INK4a und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cdkn2a/p16INK4a fusion protein Ag28927
• Vollständiger Name: cyclin-dependent kinase inhibitor 2A
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18-23 kDa
• GenBank-Zugangsnummer: NM_001040654
• Gene symbol: Cdkn2a
• Gene ID (NCBI): 12578
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cdkn2a (cyclin-dependent kinase inhibitor 2a) is a cell cycle regulating gene encoding alternate proteins, p16Ink4a and p19Arf, that arrest the G1 phase through CDK4/6 inhibition. As they are tumor suppressors, p16Ink4a and p19Arf can be targeted for treatment of various cancers, including melanoma, cervical, and esophageal cancers. The antibody is suitable for rats and mice.

Publikationen

Species	Application	Title
human,mouse	WB	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
mouse	WB	Mol Neurobiol; Hypoxic Natural Killer Cells-Derived HIF-1α-Containing Exosomes Inhibit Cellular Senescence and Apoptosis in Neurocytes to Ameliorate Alzheimer's Disease by Eliminating Oxidative Damages; Authors - Yang Zhou