p21 Polyklonaler Antikörper

p21 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28248-1-PBS

Synonyme

Cdkn1a, Cdkn1, P21 protein, p21Cip1, p21WAF

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  WB analysis using 28248-1-AP (same clone as 28248-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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  WB analysis using 28248-1-AP (same clone as 28248-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

  IP result of anti-p21 (IP:28248-1-AP, 4ug; Detection:28248-1-AP 1:1000) with NIH/3T3 cells lysate 1920 ug. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 28248-1-AP (p21 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red). This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using p21 antibody (28248-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28248-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA p21 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: p21 fusion protein Ag28394
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (P21)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: NM_001111099
• Gene symbol: p21
• Gene ID (NCBI): 12575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.