p21 Monoklonaler Antikörper

p21 Monoklonal Antikörper für IF, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Maus und mehr (2)

Anwendung

IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

3C7D3

Kat-Nr. : 67362-1-Ig

Synonyme

CAP20, CDKI, Cdkn1, Cdkn1a, CIP1, mda6, P21, p21Cip1, p21WAF, SDI1, Waf1

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Galerie der Validierungsdaten

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  IF Staining of NIH/3T3 using 67362-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 67362-1-Ig (p21 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

67362-1-Ig bindet in IF, ELISA p21 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: p21 fusion protein Ag28394
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (P21)
• Berechnetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: NM_001111099
• Gene symbol: Cdkn1a
• Gene ID (NCBI): 12575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.

Publikationen

Species	Application	Title
human,mouse		Front Pharmacol; Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B-Lamin B1 Interaction in Alcoholic Fatty Liver Disease.; Authors - Xiaoyu Qi
mouse		Life Sci; N6-methyladenosine modification on Hmbox1 is related to telomere dysfunction in DEHP-induced male reproductive injury; Authors - Xiaofang Zhu
canine	IF	Cell Prolif; TGF-β-induced PI3K/AKT/mTOR pathway controls myofibroblast differentiation and secretory phenotype of valvular interstitial cells through the modulation of cellular senescence in a naturally occurring in vitro canine model of myxomatous mitral valve disease; Authors - Qiyu Tang
mouse	IF	Heliyon; Mitochondrial citrate accumulation triggers senescence of alveolar epithelial cells contributing to pulmonary fibrosis in mice; Authors - Jie-Ru Hong