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p21 Monoklonaler Antikörper

p21 Monoklonal Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Maus

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C7D3

Kat-Nr. : 67362-1-PBS

Synonyme

Cdkn1a, p21WAF, p21Cip1, P21 protein, Cdkn1

Filter:
  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using p21 Monoclonal antibody (67362-1-Ig)

    IF Staining of NIH/3T3 using 67362-1-Ig (same clone as 67362-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 67362-1-Ig (p21 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67362-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67362-1-PBS bindet in IF/ICC, ELISA p21 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen p21 fusion protein Ag28394
Vollständiger Name cyclin-dependent kinase inhibitor 1A (P21)
Berechnetes Molekulargewicht 18 kDa
GenBank-ZugangsnummerNM_001111099
Gene symbol p21
Gene ID (NCBI) 12575
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.