p300 Rekombinanter Antikörper

p300 Rekombinant Antikörper für IF/ICC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

230369D12

Kat-Nr. : 83078-5-PBS

Synonyme

EP300, 230369D12, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48

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  IP experiment of HeLa using 83078-5-RR (same clone as 83078-5-PBS)

  IP result of anti-p300 (IP:83078-5-RR, 4ug; Detection:83078-5-RR 1:1000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83078-5-RR (same clone as 83078-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83078-5-RR (same clone as 83078-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 83078-5-RR (same clone as 83078-5-PBS)

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

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  ChIP experiment of K-562 using 83078-5-RR (same clone as 83078-5-PBS)

  Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of p300 (83078-5-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83078-5-PBS bindet in IF/ICC, FC (Intra), IP, Indirect ELISA, ChIP-qPCR p300 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: E1A binding protein p300
• Berechnetes Molekulargewicht: 264 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: NM_001429
• Gene symbol: EP300
• Gene ID (NCBI): 2033
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).