AMACR/p504S Polyklonaler Antikörper

AMACR/p504S Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 15918-1-AP

Synonyme

AMACR, p504S, EC:5.1.99.4, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase

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  WB analysis using 15918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15918-1-AP (AMACR/p504S antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15918-1-AP (AMACR/p504S antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human prostate cancer using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15918-1-AP (AMACR/p504S antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human renal cell carcinoma using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15918-1-AP (AMACR/p504S antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15918-1-AP (AMACR/p504S antibody at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

15918-1-AP bindet in WB, IHC, IF, ELISA AMACR/p504S und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMACR/p504S fusion protein Ag8720
• Vollständiger Name: alpha-methylacyl-CoA racemase
• Berechnetes Molekulargewicht: 382 aa, 42 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC009471
• Gene symbol: AMACR
• Gene ID (NCBI): 23600
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing and it can form a dimer of 70-75 kDa(PMID:21812041). Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	IHC	Front Cell Dev Biol; SMARCC1 Suppresses Tumor Progression by Inhibiting the PI3K/AKT Signaling Pathway in Prostate Cancer.; Authors - Zhao-Ming Xiao
human	IHC	Oncol Lett; The auxiliary diagnostic value of prostate-specific antigen and α-methylacyl-CoA racemase in prostate cancer.; Authors - Daijun Yang
human	IHC	Andrologia; Circ_0076305 facilitates prostate cancer development via sponging miR-411-5p and regulating PGK1.; Authors - Xiqi Ding
human	WB,IF	Commun Biol; ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer; Authors - Rebecca Garnham