AMACR/p504S Monoklonaler Antikörper

AMACR/p504S Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H9B5

Kat-Nr. : 60240-1-PBS

Synonyme

AMACR, p504S, EC:5.1.99.4, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase

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  WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60240-1-Ig (same clone as 60240-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60240-1-Ig (same clone as 60240-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using p504S,AMACR antibody (60240-1-Ig, Clone: 2H9B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60240-1-PBS bindet in WB, IF/ICC, Indirect ELISA AMACR/p504S und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AMACR/p504S fusion protein Ag8720
• Vollständiger Name: alpha-methylacyl-CoA racemase
• Berechnetes Molekulargewicht: 382 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC009471
• Gene symbol: AMACR
• Gene ID (NCBI): 23600
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).