AMACR/p504S Rekombinanter Antikörper

AMACR/p504S Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242456D1

Kat-Nr. : 84891-2-PBS

Synonyme

AMACR, p504S, 242456D1, 2-methylacyl-CoA racemase, Alpha-methylacyl-CoA racemase

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  WB analysis using 84891-2-RR (same clone as 84891-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84891-2-RR (AMACR/p504S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84891-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 84891-2-RR (same clone as 84891-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84891-2-RR (AMACR/p504S antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84891-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84891-2-PBS bindet in WB, IHC, Indirect ELISA AMACR/p504S und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AMACR/p504S fusion protein Ag8720
• Vollständiger Name: alpha-methylacyl-CoA racemase
• Berechnetes Molekulargewicht: 382 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC009471
• Gene symbol: AMACR
• Gene ID (NCBI): 23600
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. A mitochondrial and peroxisomal enzyme catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing and can form a dimer of 70-75 kDa(PMID:21812041). Defects in AMACR cause alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).