p57Kip2 Monoklonaler Antikörper

p57Kip2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A12H3

Kat-Nr. : 66794-1-PBS

Synonyme

CDKN1C, KIP2, p57, Cyclin-dependent kinase inhibitor p57, Cyclin-dependent kinase inhibitor 1C

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  WB analysis using 66794-1-Ig (same clone as 66794-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66794-1-Ig (CDKN1C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

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  WB analysis using 66794-1-Ig (same clone as 66794-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66794-1-Ig (p57Kip2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66794-1-Ig (same clone as 66794-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66794-1-Ig (CDKN1C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66794-1-Ig (same clone as 66794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66794-1-Ig (p57Kip2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66794-1-Ig (same clone as 66794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66794-1-Ig (p57Kip2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66794-1-PBS bindet in WB, IHC, Indirect ELISA p57Kip2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: p57Kip2 fusion protein Ag19927
• Vollständiger Name: cyclin-dependent kinase inhibitor 1C (p57, Kip2)
• Berechnetes Molekulargewicht: 316 aa, 32 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC067842
• Gene symbol: p57 Kip2/CDKN1C
• Gene ID (NCBI): 1028
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1C, also named as p57KIP2 and KIP2, belongs to the CDI family. CDKN1C is a potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. It is a negative regulator of cell proliferation. CDKN1C may play a role in maintenance of the non-proliferative state throughout life.