p70(S6K) Polyklonaler Antikörper

p70(S6K) Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 26587-1-AP

Synonyme

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6k, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A

---

Galerie der Validierungsdaten

• 

  WB analysis of MCF-7 using 26587-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26587-1-AP (p70(S6K) Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26587-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26587-1-AP (p70(S6K) Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 26587-1-AP

  IP Result of anti-p70(S6K) (IP:26587-1-AP, 4ug; Detection:26587-1-AP 1:300) with A549 cells lysate 1600ug.

• 

  IHC staining of human lung cancer using 26587-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 26587-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 26587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 26587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26587-1-AP (p70(S6K) Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 26587-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26587-1-AP (p70(S6K) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

26587-1-AP bindet in WB, IP, IHC, IF, ELISA p70(S6K) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: p70(S6K) fusion protein Ag24073
• Vollständiger Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 60-75 kDa
• GenBank-Zugangsnummer: BC053365
• Gene symbol: RPS6KB1
• Gene ID (NCBI): 6198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59kDa produced by alternative initiation.

Publikationen

Species	Application	Title
pig	WB	Am J Physiol Endocrinol Metab; Prematurity blunts the feeding-induced stimulation of translation initiation signaling and protein synthesis in muscle of neonatal piglets.; Authors - Jane K Naberhuis