pan Ras Monoklonaler Antikörper

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• KD/KO Validated

pan Ras Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4H4G7

Kat-Nr. : 60309-1-PBS

Synonyme

KRAS, 4H4G7, C K RAS, c Ki ras, c-Ki-ras

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  WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  WB analysis using 60309-1-Ig (same clone as 60309-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60309-1-Ig (same clone as 60309-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 60309-1-Ig (same clone as 60309-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 60309-1-Ig (same clone as 60309-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60309-1-Ig (pan Ras Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 60309-1-Ig (same clone as 60309-1-PBS)

  IP result of anti-pan Ras (IP:60309-1-Ig, 5ug; Detection:60309-1-Ig 1:2000) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60309-1-Ig (pan Ras antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60309-1-Ig (pan Ras Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 60309-1-Ig (same clone as 60309-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using pan Ras antibody (60309-1-Ig, Clone: 4H4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 60309-1-Ig (same clone as 60309-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human pan Ras (60309-1-Ig, Clone:4H4G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60309-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50398-1

  Cytometric bead array standard curve of MP50398-1, KRAS Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60309-2-PBS. Detection antibody: 60309-1-PBS. Standard:Ag2700. Range: 0.781-12.5 ng/mL.

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  Cytometric bead array standard curve of MP80009-1

  Cytometric bead array standard curve of MP80009-1, KRAS Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 60309-1-PBS. Detection antibody: 81615-1-PBS. Standard: Ag2700. Range: 0.391-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

60309-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA pan Ras und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: pan Ras fusion protein Ag2700
• Vollständiger Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 188 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC013572
• Gene symbol: KRAS
• Gene ID (NCBI): 3845
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.