pan-keratin Rekombinanter Antikörper

pan-keratin Rekombinant Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

7J23

Kat-Nr. : 82548-3-RR

Synonyme

CK-PAN, pan Cytokeratin, pan keratin, pan-Cytokeratin, pan-keratin

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  IHC staining of human lung cancer using 82548-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 82548-3-RR (KRT5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82548-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 82548-3-RR (KRT5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82548-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 82548-3-RR (KRT5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82548-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 82548-3-RR (KRT5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 82548-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KRT5 antibody (82548-3-RR, Clone: 7J23 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 82548-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRT5 antibody (82548-3-RR, Clone: 7J23 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82548-3-RR bindet in IF, IHC, ELISA pan-keratin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: pan-keratin fusion protein Ag24184
• Vollständiger Name: keratin 5
• Berechnetes Molekulargewicht: 590 aa, 62 kDa
• GenBank-Zugangsnummer: BC024292
• Gene symbol: Cytokeratin 5
• Gene ID (NCBI): 3852
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.