Peroxiredoxin 2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Peroxiredoxin 2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H6C4

Kat-Nr. : 60202-1-PBS

Synonyme

PRDX2, 3H6C4, EC:1.11.1.24, Natural killer cell-enhancing factor B, NKEFB

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  WB analysis using 60202-1-Ig (same clone as 60202-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60202-1-Ig (same clone as 60202-1-PBS)

  WB result of Peroxiredoxin 2 antibody (60202-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60202-1-Ig (same clone as 60202-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60202-1-Ig (same clone as 60202-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(Peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(Peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(Peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Peroxiredoxin 2 antibody (60202-1-Ig, Clone: 3H6C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60202-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Peroxiredoxin 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peroxiredoxin 2 fusion protein Ag0835
• Vollständiger Name: peroxiredoxin 2
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC003022
• Gene symbol: peroxiredoxin 2
• Gene ID (NCBI): 7001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)