persephin-Specific Polyklonaler Antikörper

persephin-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19713-1-PBS

Synonyme

persephin, PSPN, PSP

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  WB analysis using 19713-1-AP (same clone as 19713-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 19713-1-AP (same clone as 19713-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19713-1-AP (same clone as 19713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 19713-1-AP (same clone as 19713-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using persephin-Specific antibody (19713-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 19713-1-AP (same clone as 19713-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 19713-1-AP (same clone as 19713-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 19713-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19713-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA persephin-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: persephin
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: NM_004158
• Gene symbol: Persephin/PSPN
• Gene ID (NCBI): 5623
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSPN, also named as PSP, belongs to the TGF-beta family and GDNF subfamily. PSPN exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. The antibody is specific to PSPN.