Phospho-TDP43 (Ser403/404) Monoklonaler Antikörper
Phospho-TDP43 (Ser403/404) Monoklonal Antikörper für WB, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
6B11B12
Kat-Nr. : 66079-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, fetales humanes Hirngewebe, Jurkat-Zellen, K-562-Zellen, RAW 264.7-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
Produktinformation
66079-1-Ig bindet in WB, IHC, IF, ELISA Phospho-TDP43 (Ser403/404) und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| In Publikationen genannte Reaktivität | human, Maus, Zebrafisch |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | TAR DNA binding protein |
| Berechnetes Molekulargewicht | 43 kDa |
| Beobachtetes Molekulargewicht | 25 kDa |
| GenBank-Zugangsnummer | NM_007375 |
| Gene symbol | TDP-43 |
| Gene ID (NCBI) | 23435 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).66079-1-Ig is a mouse monoclonal antibody recognizing TDP-43 only when phosphorylated at 403/404. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Phospho-TDP43 (Ser403/404) antibody 66079-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Neurosci TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury | ||
EMBO J Disease-linked TDP-43 hyperphosphorylation suppresses TDP-43 condensation and aggregation. | ||
Eur J Med Chem Targeting nuclear protein TDP-43 by cell division cycle kinase 7 inhibitors: A new therapeutic approach for amyotrophic lateral sclerosis. | ||
Biochemistry Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis. | ||
PLoS One Drosophila lines with mutant and wild type human TDP-43 replacing the endogenous gene reveals phosphorylation and ubiquitination in mutant lines in the absence of viability or lifespan defects. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by Phospho-TDP43 (Ser403/404) Monoclonal antibody at dilution of 1:100 showed strong labelling.
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