Phospho-TDP43 (Ser409/410) Rekombinanter Antikörper

Phospho-TDP43 (Ser409/410) Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

CloneNo.

6M10

Rezensionen (2)

Kat-Nr. : 80007-1-RR

Synonyme

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43

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  WB analysis using 80007-1-RR

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80007-1-RR (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

• 

  IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR

  IHC results of Phospho-TDP43 (Ser409/410) rabbit recombinant antibody (80007-1-RR, 1000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann.

• 

  IHC staining of human brain using 80007-1-RR

  It's human subiculum (high-mag) and temporal cortex (medium-mag) from subject with limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC). Staining provided by Pete Nelson and Ela Patel, U. Kentucky AD Research Center Neuropathology Core.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Calyculin A treated cells
• Erfolgreiche Detektion in IHC: frontaler Cortex eines FTLD-TDP Typ B-Falls, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 8 publications below
• IF: See 10 publications below

Produktinformation

80007-1-RR bindet in WB, IHC, IF, ELISA Phospho-TDP43 (Ser409/410) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TAR DNA binding protein
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: NM_007375
• Gene symbol: TDP-43
• Gene ID (NCBI): 23435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Publikationen

Species	Application	Title
mouse	WB	J Biol Chem; Regulation of TAR DNA binding protein 43 (TDP-43) homeostasis by cytosolic DNA accumulation; Authors - Cha Yang
	WB	Biochim Biophys Acta Mol Basis Dis; Phosphorylated TAR DNA-Binding Protein-43: Aggregation and Antibody-Based Inhibition.; Authors - Josephine C Esposto
human	WB,IF	bioRxiv; TDP-43 pathology links innate and adaptive immunity in amyotrophic lateral sclerosis; Authors - Baggio A Evangelista
human,mouse	IF	bioRxiv; Fructose-2,6-bisphosphate restores TDP-43 pathology-driven genome repair deficiency in motor neuron diseases; Authors - Anirban Chakraborty
mouse	WB,IF	Ecotoxicol Environ Saf; Arsenic exposure activates microglia, inducing neuroinflammation and promoting the occurrence and development of Alzheimer's disease-like neurodegeneration in mice; Authors - Bo Zhang
mouse	IF	Res Sq; Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence; Authors - Joy Mitra

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rashmi (Verified Customer) (02-14-2025)

used for Western blot, IHC, highly recommended

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 , 1:2000
• Cell Tissue Type: HEK293T

FH

Silvia (Verified Customer) (08-21-2020)

Excellent phospho-specific TDP-43 antibody. Tested by IHC in ethanol and NBF fixed FTLD-TDP brains and ALS spinal cord. The antibody works well with citrate buffer and FA as antigen retrieval. The dilutions that worked better showing high specificity and low background ranged from 1,000-5,000.Works great also by western blot at dilutions 1:1,000-1:2,000.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000)
• Cell Tissue Type: human cortex and spinal cord

Phospho-TDP43 (Ser409/410) Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000) dilution) in human cortex and spinal cord (Cat no:80007-1-RR)