Alpha smooth muscle actin Polyklonaler Antikörper

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• KD/KO Validated

Alpha smooth muscle actin Polyklonal Antikörper für IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(5)

Kat-Nr. : CL488-14395

Synonyme

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

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  IF Staining of mouse heart using CL488-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL488-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200, CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Mausherzgewebe, Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 5 publications below

Produktinformation

CL488-14395 bindet in IF-P Alpha smooth muscle actin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha smooth muscle actin fusion protein Ag5886
• Vollständiger Name: actin, alpha 2, smooth muscle, aorta
• Berechnetes Molekulargewicht: 377 aa, 42 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC017554
• Gene symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Publikationen

Species	Application	Title
mouse	IF	Cell Metab; Myocardial infarction accelerates the progression of MASH by triggering immunoinflammatory response and induction of periosti; Authors - Wei Xie
	IF	Nat Commun; ACSS2 protects against alcohol-induced hepatocyte ferroptosis through regulation of hepcidin expression; Authors - Mengyao Wang
mouse	IF	Front Med; Targeting deubiquitinase OTUB1 protects vascular smooth muscle cells in atherosclerosis by modulating PDGFRβ; Authors - Fei Xu
	IF	Diabet Med; Mouse islet-derived stellate cells are similar to, but distinct from, mesenchymal stromal cells and influence the beta cell function; Authors - Wei Xu
rat	IF	Regen Biomater; Magnetic fibrin nanofiber hydrogel delivering iron oxide magnetic nanoparticles promotes peripheral nerve regeneration; Authors - Juncong Hong