Alpha smooth muscle actin specific Monoklonaler Antikörper

Alpha smooth muscle actin specific Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1E9A11

Kat-Nr. : 67735-1-PBS

Synonyme

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 1E9A11

---

• 

  WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67735-1-Ig (same clone as 67735-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig vein using 67735-1-Ig (same clone as 67735-1-PBS)

  pig vein tissue were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat heart using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using a-SMA specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse breast cancer using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Alpha smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 67735-1-Ig (same clone as 67735-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

• 

  FC experiment of C2C12 using 67735-1-Ig (same clone as 67735-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human smooth muscle actin specific (67735-1-Ig, Clone:1E9A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67735-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67735-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA Alpha smooth muscle actin specific und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: actin, alpha 2, smooth muscle, aorta
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_001613
• Gene symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.