SARS-CoV-2 S protein (944-1214 aa) Polyklonaler Antikörper

SARS-CoV-2 S protein (944-1214 aa) Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus und mehr (3)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 28867-1-AP

Synonyme

RBD, COVID-19 RBD, Spike, spike protein, SARS-CoV-2 Spike

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  ELISA experiment of Recombinant protein using 28867-1-AP

  SARS-CoV-2 Spike Antibody (28867-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (28867-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in ELISA: Recombinant protein

Empfohlene Verdünnung

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 8 publications below

Produktinformation

28867-1-AP bindet in WB, IF, ELISA SARS-CoV-2 S protein (944-1214 aa) und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• In Publikationen genannte Reaktivität: human, Affe, hamster
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARS-CoV-2 S protein (944-1214 aa) fusion protein Ag30685
• Vollständiger Name: SARS-CoV-2 Spike Protein
• Berechnetes Molekulargewicht: 141 kDa
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: SARS-COV-2
• Gene ID (NCBI): 43740568
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). This antibody detects the spike protein of SARS and SARS-COV-2.

Publikationen

Species	Application	Title
monkey,hamster	IF	Nat Biomed Eng; High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2; Authors - Charles W F Chan
human	IF	Nat Immunol; Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules.; Authors - Matteo Stravalaci
human	IF	Nat Commun; Essential role of TMPRSS2 in SARS-CoV-2 infection in murine airways; Authors - Naoko Iwata-Yoshikawa
	WB	bioRxiv; Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus; Authors - Qiwang Xiang
	IF	Cell Rep; Diverse immunoglobulin gene usage and convergent epitope targeting in neutralizing antibody responses to SARS-CoV-2.; Authors - Xiaojuan Zhou
human	IF	J Virol; The respective roles of TMPRSS2 and cathepsins for SARS-CoV-2 infection in human respiratory organoids; Authors - Masatoshi Kakizaki