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SARS-CoV-2 Spike Rekombinanter Antikörper

SARS-CoV-2 Spike Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4I18

Kat-Nr. : 82734-4-PBS

Synonyme

COVID-19 RBD, RBD, Spike, spike protein, S protein

Filter:
  • Western Blot (WB) analysis of Recombinant protein using SARS-CoV-2 Spike Recombinant antibody (82734-4-RR)

    WB analysis of Recombinant protein using 82734-4-RR (same clone as 82734-4-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 82734-4-RR (spike protein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82734-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82734-4-RR

    Affinity and Kinetic Characterization of 82734-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82734-4-RR against Virus spike protein were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

82734-4-PBS bindet in WB, Indirect ELISA SARS-CoV-2 Spike und zeigt Reaktivität mit Virus

Getestete Reaktivität Virus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen SARS-CoV-2 Spike fusion protein Ag30684
Vollständiger Name SARS-CoV-2 Spike Protein
Berechnetes Molekulargewicht 141 kDa
GenBank-ZugangsnummerNC_045512
Gene symbol SARS-COV-2
Gene ID (NCBI) 43740568
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.