SARS-CoV-2 Spike Rekombinanter Antikörper

SARS-CoV-2 Spike Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

4I18

Kat-Nr. : 82734-4-RR

Synonyme

COVID-19 RBD, RBD, Spike, spike protein, S protein

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  WB analysis of Recombinant protein using 82734-4-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 82734-4-RR (spike protein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82734-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82734-4-RR against Virus spike protein were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82734-4-RR bindet in WB, ELISA SARS-CoV-2 Spike und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SARS-CoV-2 Spike fusion protein Ag30684
• Vollständiger Name: SARS-CoV-2 Spike Protein
• Berechnetes Molekulargewicht: 141 kDa
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: SARS-COV-2
• Gene ID (NCBI): 43740568
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.