transgelin/SM22 Monoklonaler Antikörper

transgelin/SM22 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A10C2

Kat-Nr. : 60213-1-PBS

Synonyme

SM22, transgelin, 22 kDa actin-binding protein, 2A10C2, Protein WS3-10

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  WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig stomach using 60213-1-Ig (same clone as 60213-1-PBS)

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 60213-1-Ig (same clone as 60213-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat stomach using 60213-1-Ig (same clone as 60213-1-PBS)

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human stomach using 60213-1-Ig (same clone as 60213-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (60213-1-Ig, Clone: 2A10C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60213-1-PBS bindet in WB, IHC, IF-P, ELISA transgelin/SM22 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: transgelin/SM22 fusion protein Ag0764
• Vollständiger Name: transgelin
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 18+22 kDa
• GenBank-Zugangsnummer: BC004927
• Gene symbol: transgelin/SM22
• Gene ID (NCBI): 6876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.