uPAR/CD87 Rekombinanter Antikörper

uPAR/CD87 Rekombinant Antikörper für IF/ICC, FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC

Konjugation

Unkonjugiert

CloneNo.

240366G10

Kat-Nr. : 98048-1-RR

Synonyme

PLAUR, UPAR, U PAR, MO3, CD87

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  IF Staining of HeLa using 98048-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using uPAR/CD87 antibody (98048-1-RR, Clone: 240366G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U-937 using 98048-1-RR

  1x10^6 PMA-treated U-937 cells were surface stained with 0.25 ug Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (98048-1-RR, Clone: 240366G10) (red) or isotype control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Untreated U-937 cells were surface stained with 0.25 ug Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (98048-1-RR, Clone: 240366G10) and PE-conjugated Goat Anti-Rabbit IgG (black). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98048-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98048-1-RR against Human uPAR were performed. The affinity constant is 4.70 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC: PMA treated U-937 cells

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

98048-1-RR bindet in IF/ICC, FC uPAR/CD87 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: PLAUR
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: NM_002659.4
• Gene symbol: uPAR
• Gene ID (NCBI): 5329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

uPAR, also known as CD87, is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)