uPAR/CD87 Monoklonaler Antikörper

uPAR/CD87 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A3E8

Kat-Nr. : 68727-1-Ig

Synonyme

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

---

• 

  WB analysis of U2OS using 68727-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68727-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR, PLAUR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung squamous cell cancer using 68727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung squamous cell cancer using 68727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A549-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: human lung squamous cell cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68727-1-Ig bindet in WB, IHC, ELISA uPAR/CD87 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: uPAR/CD87 fusion protein Ag28056
• Vollständiger Name: PLAUR
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35-50 kDa
• GenBank-Zugangsnummer: BC002788
• Gene symbol: uPAR
• Gene ID (NCBI): 5329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)