uPAR/CD87 Monoklonaler Antikörper

uPAR/CD87 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A3E8

Kat-Nr. : 68727-1-PBS

Synonyme

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

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  WB analysis of U2OS using 68727-1-Ig (same clone as 68727-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68727-1-Ig (same clone as 68727-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR, PLAUR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung squamous cell cancer using 68727-1-Ig (same clone as 68727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung squamous cell cancer using 68727-1-Ig (same clone as 68727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68727-1-PBS bindet in WB, IHC, Indirect ELISA uPAR/CD87 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: uPAR/CD87 fusion protein Ag28056
• Vollständiger Name: PLAUR
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35-50 kDa
• GenBank-Zugangsnummer: BC002788
• Gene symbol: uPAR
• Gene ID (NCBI): 5329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)