uPAR/CD87 Rekombinanter Antikörper

uPAR/CD87 Rekombinant Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230148A1

Kat-Nr. : 82953-1-PBS

Synonyme

uPAR, PLAUR, PLAUR, 230148A1, CD87, MO3

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  IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 82953-1-RR (same clone as 82953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82953-1-RR (same clone as 82953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-1080 using 82953-1-RR (same clone as 82953-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using uPAR/CD87 antibody (82953-1-RR, Clone: 230148A1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82953-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82953-1-RR against Human uPAR, PLAUR were performed. The affinity constant is 1.49 nM.

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Geprüfte Anwendungen

Produktinformation

82953-1-PBS bindet in IHC, IF/ICC, Indirect ELISA uPAR/CD87 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: uPAR/CD87 fusion protein Ag28056
• Vollständiger Name: PLAUR
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC002788
• Gene symbol: uPAR
• Gene ID (NCBI): 5329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)