uPAR/CD87 Rekombinanter Antikörper

uPAR/CD87 Rekombinant Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230148A1

Kat-Nr. : 82953-1-RR

Synonyme

uPAR, PLAUR, PLAUR, 230148A1, CD87, MO3

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  IHC staining of mouse brain using 82953-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82953-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 82953-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 82953-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-1080 using 82953-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using uPAR/CD87 antibody (82953-1-RR, Clone: 230148A1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 82953-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82953-1-RR against Human uPAR, PLAUR were performed. The affinity constant is 1.49 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HT-1080.Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82953-1-RR bindet in IHC, IF/ICC, ELISA uPAR/CD87 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: uPAR/CD87 fusion protein Ag28056
• Vollständiger Name: PLAUR
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC002788
• Gene symbol: uPAR
• Gene ID (NCBI): 5329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)