ubiquitin Monoklonaler Antikörper

ubiquitin Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1D7B2

Kat-Nr. : 60310-1-Ig

Synonyme

ubiquitin

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 60310-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60310-1-Ig (ubiquitin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 60310-1-Ig

  Immunofluorescent analysis of HeLa cells using 60310-1-Ig (ubiquitin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

60310-1-Ig bindet in WB, IHC, IF, ELISA ubiquitin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ubiquitin fusion protein Ag0260
• Vollständiger Name: ubiquitin B
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC000379
• Gene symbol: UBB
• Gene ID (NCBI): 7314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.

Publikationen

Species	Application	Title
human		Cancer Lett; Exosome-mediated transfer of SNHG7 enhances docetaxel resistance in lung adenocarcinoma; Authors - Kai Zhang
	WB	Cell Death Differ; Parkin maintains mitochondrial levels of the protective Parkinson's disease-related enzyme 17-β hydroxysteroid dehydrogenase type 10.; Authors - G Bertolin
human	WB	Autophagy; Sensitive ELISA-based detection method for the mitophagy marker p-S65-Ub in human cells, autopsy brain, and blood samples; Authors - Jens O. Watzlawik