ubiquitin Rekombinanter Antikörper

ubiquitin Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, Hefe, human, hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6H6

Kat-Nr. : 80992-1-PBS

Synonyme

UBB, ubiquitin B, 6H6, Polyubiquitin B, Polyubiquitin-B

---

• 

  WB analysis using 80992-1-RR (same clone as 80992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80992-1-RR (ubiquitin antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 80992-1-RR (same clone as 80992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 80992-1-RR (same clone as 80992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 80992-1-RR (same clone as 80992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 80992-1-RR (same clone as 80992-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ubiquitin antibody (80992-1-RR, Clone: 6H6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80992-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80992-1-RR against Human ubiquitin were performed. The affinity constant is 1.57 nM.

---

Geprüfte Anwendungen

Produktinformation

80992-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ubiquitin und zeigt Reaktivität mit hamster, Hefe, human, hund, Maus, Ratten

• Getestete Reaktivität: hamster, Hefe, human, hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ubiquitin fusion protein Ag0260
• Vollständiger Name: ubiquitin B
• GenBank-Zugangsnummer: BC000379
• Gene symbol: ubiquitin
• Gene ID (NCBI): 7314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.