ubiquitin Rekombinanter Antikörper

ubiquitin Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, Hefe, human, hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

CloneNo.

6H6

Kat-Nr. : 80992-1-RR

Synonyme

UBB, ubiquitin B, 6H6, Polyubiquitin B, Polyubiquitin-B

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  WB analysis using 80992-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80992-1-RR (ubiquitin antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 80992-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 80992-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 80992-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 80992-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ubiquitin antibody (80992-1-RR, Clone: 6H6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 80992-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80992-1-RR against Human ubiquitin were performed. The affinity constant is 1.57 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, mit MG132 behandelte NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:100000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below

Produktinformation

80992-1-RR bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA ubiquitin und zeigt Reaktivität mit hamster, Hefe, human, hund, Maus, Ratten

• Getestete Reaktivität: hamster, Hefe, human, hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ubiquitin fusion protein Ag0260
• Vollständiger Name: ubiquitin B
• GenBank-Zugangsnummer: BC000379
• Gene symbol: ubiquitin
• Gene ID (NCBI): 7314
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.

Publikationen

Species	Application	Title
human	WB	Cell Signal; Repurposing a plant alkaloid homoharringtonine targets insulinoma associated-1 in N-Myc-activated neuroblastoma; Authors - Chiachen Chen
human	IP	Clin Transl Oncol; LncRNA XXYLT1-AS2 promotes tumor progression via autophagy inhibition through ubiquitinated degradation of TFEB in hepatocellular carcinoma; Authors - Xuejie Li
mouse	WB,IP	Free Radic Biol Med; Carnitine functions as an enhancer of NRF2 to inhibit osteoclastogenesis via regulating macrophage polarization in osteoporosis; Authors - Tao Yang
mouse	WB,IP	Biochem Pharmacol; BCL6 confers resistance to HDAC inhibitors in DLBCL; Authors - Gao Fan
human	WB	iScience; CircEZH2 promotes gallbladder cancer progression and lipid metabolism reprogramming through the miR-556-5p/SCD1 axis; Authors - Huanjun Tong
human	IP	Sci Rep; FBXO2 promotes the progression of papillary thyroid carcinoma through the p53 pathway; Authors - Wenke Guo