VWF Rekombinanter Antikörper

VWF Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240867D10

Kat-Nr. : 83854-2-PBS

Synonyme

VWF, VWFpp, 240867D10, F8VWF, von Willebrand antigen II, VWD

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  WB analysis using 83854-2-RR (same clone as 83854-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83854-2-RR (vwf antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83854-2-RR (same clone as 83854-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83854-2-RR (same clone as 83854-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83854-2-RR (same clone as 83854-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83854-2-RR (same clone as 83854-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 83854-2-RR (same clone as 83854-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VWF antibody (83854-2-RR, Clone: 240867D10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 83854-2-RR (same clone as 83854-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VWF antibody (83854-2-RR, Clone: 240867D10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83854-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83854-2-PBS against Human VWF was performed. The affinity constant is 0.605 nM.

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  Affinity and Kinetic Characterization of 83854-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83854-2-RR against Human VWF were performed. The affinity constant is 0.965 nM.

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Geprüfte Anwendungen

Produktinformation

83854-2-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA VWF und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VWF fusion protein Ag25578
• Vollständiger Name: von Willebrand factor
• Beobachtetes Molekulargewicht: 309-320 kDa
• Gene symbol: VWF
• Gene ID (NCBI): 7450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).