Anticorps Monoclonal anti-1-Methyladenosine

1-Methyladenosine Monoclonal Antibody for Dot Blot, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

chemical compound

Applications

Dot Blot, ELISA

Conjugaison

Non conjugué

CloneNo.

2F2H8

N° de cat : 68636-1-Ig

Synonymes

N1-methyladenosine, m1A

---

Galerie de données de validation

• 

  Dot Blot experiment of RNA using 68636-1-Ig

  Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose.

---

Applications testées

• Résultats positifs en Dot Blot: RNA,

Dilution recommandée

• DOT BLOT: DOT BLOT : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68636-1-Ig cible 1-Methyladenosine dans les applications de Dot Blot, ELISA et montre une réactivité avec des échantillons chemical compound

• Réactivité: chemical compound
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTG
• Nom complet: 1-Methyladenosine
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf

{{ptg:RelatedPrimaryAntibodies}}