Anticorps Polyclonal de lapin anti-14-3-3

• Phare
• Validé par KD/KO

14-3-3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 14503-1-AP

Synonymes

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 1C5, HS1, Protein HS1, YWHAQ

---

Galerie de données de validation

• 

  WB analysis using 14503-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14503-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14503-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14503-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14503-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14503-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 14503-1-AP

  IP Result of anti-14-3-3 (IP:14503-1-AP, 5ug; Detection:14503-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14503-1-AP

  Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 14503-1-AP

  Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 14503-1-AP

  1X10^6 HeLa cells were stained with 0.2ug 14-3-3 antibody (14503-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules Jurkat, cellules NIH/3T3, tissu cardiaque de souris, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

14503-1-AP cible 14-3-3 dans les applications de WB, RIP, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: 14-3-3 Protéine recombinante Ag5959
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC056867
• Symbole du gène: YWHAQ
• Identification du gène (NCBI): 10971
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

Publications

Species	Application	Title
		Int J Biol Macromol; Biofilm formation initiating rotifer-specific biopolymer and its predicted components; Authors - Zsolt Datki
human	WB	Elife; Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding.; Authors - Rosalind Norkett ; KD Validated
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human,mouse	WB,IP	Aging (Albany NY); The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.; Authors - Chunyan Li
human	WB	J Inflamm Res; PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles.; Authors - Bo Huang
rat	IP	Biochim Biophys Acta Mol Basis Dis; Nedd4-2 haploinsufficiency in mice causes increased seizure susceptibility and impaired Kir4.1 ubiquitination.; Authors - Xiaoliang Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Renal Cancer cell line