Anticorps Monoclonal anti-14-3-3

14-3-3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

2E10A3

N° de cat : 66061-1-Ig

Synonymes

YWHAQ, 14-3-3 protein T-cell, 14-3-3 theta, 14-3-3 zeta, 14-3-3σ

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Galerie de données de validation

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  WB analysis using 66061-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 66061-1-Ig

  IP result of anti-14-3-3 (IP:66061-1-Ig, 4ug; Detection:66061-1-Ig 1:20000) with rat brain tissue lysate 1080 ug.

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  IHC staining of human lung cancer using 66061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 66061-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat brain using 66061-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 66061-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de rat,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66061-1-Ig cible 14-3-3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: 14-3-3 Protéine recombinante Ag6055
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 31 kDa, 28 kDa
• Numéro d’acquisition GenBank: BC056867
• Symbole du gène: 14-3-3 theta
• Identification du gène (NCBI): 10971
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

Publications

Species	Application	Title
mouse	IHC	Aging (Albany NY); In vitro and in vivo efficacy of the novel oral proteasome inhibitor NNU546 in multiple myeloma.; Authors - Hui Zhou
human	IF	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos
rat,human	WB,CoIP	Neurosci Res; HAP1 interacts with 14-3-3 to regulate epileptic seizure via GABAAR-mediated inhibitory synaptic transmission in pentylenetetrazole rat model.; Authors - Yuetao Wen
mouse	WB,IP	Clin Nutr; Ceramide enhanced the hepatic glucagon response through regulation of CREB activity; Authors - Jizheng Wang
pig	WB	J Virol; The African swine fever virus MGF360-16R protein functions as a mitochondrial-dependent apoptosis inducer by competing with BAX to bind to the HSP60 protein; Authors - Zhiyong Xiang

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