Anticorps Monoclonal anti-14-3-3 Sigma

14-3-3 Sigma Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F10D9

N° de cat : 66251-1-PBS

Synonymes

14-3-3 protein sigma, 14-3-3sigma, 3F10D9, HME1, SFN

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Galerie de données de validation

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  WB analysis using 66251-1-Ig (same clone as 66251-1-PBS)

  Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  WB analysis of A-253 using 66251-1-Ig (same clone as 66251-1-PBS)

  A-253 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 Sigma antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66251-1-Ig (same clone as 66251-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66251-1-Ig (same clone as 66251-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14-3-3 Sigma antibody (66251-1-Ig, Clone: 3F10D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66251-1-PBS cible 14-3-3 Sigma dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: 14-3-3 Sigma Protéine recombinante Ag12644
• Nom complet: stratifin
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC002995
• Symbole du gène: 14-3-3 Sigma
• Identification du gène (NCBI): 2810
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

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