Anticorps Polyclonal de lapin anti-14-3-3 Epsilon

• Phare
• Validé par KD/KO

14-3-3 Epsilon Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(22)

N° de cat : 11648-2-AP

Synonymes

14-3-3E, YWHAE, 14-3-3 protein epsilon, 14-3-3epsilon

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Galerie de données de validation

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  WB analysis of A375 using 11648-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11648-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 11648-2-AP

  IP result of anti-14-3-3 Epsilon (IP:11648-2-AP, 3ug; Detection:11648-2-AP 1:1000) with A375 cells lysate 6000ug.

• 

  IHC staining of human colon using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11648-2-AP (14-3-3 Epsilon antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11648-2-AP (14-3-3 Epsilon antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11648-2-AP (14-3-3 epsilon antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 11648-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14-3-3 Epsilon antibody (11648-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HeLa
• Résultats positifs en IP: cellules A375
• Résultats positifs en IHC: tissu de côlon humain, tissu cérébral de souris, tissu de cancer du poumon humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IF: See 6 publications below
• IP: See 3 publications below

Informations sur le produit

11648-2-AP cible 14-3-3 Epsilon dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: 14-3-3 Epsilon Protéine recombinante Ag2247
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Masse moléculaire calculée: 255 aa, 29 kDa
• Poids moléculaire observé: 29-32 kDa
• Numéro d’acquisition GenBank: BC000179
• Symbole du gène: 14-3-3E
• Identification du gène (NCBI): 7531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

14-3-3 Epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 Epsilon.

Publications

Species	Application	Title
human	WB,IF	Nat Immunol; A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.; Authors - Ying Kai Chan
human	WB	Nat Commun; Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation; Authors - Jiayu Liu
human	IP	Cell Host Microbe; Zika Virus NS3 Mimics a Cellular 14-3-3-Binding Motif to Antagonize RIG-I- and MDA5-Mediated Innate Immunity.; Authors - William Riedl
mouse	WB	EMBO J; AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.; Authors - Xin Zheng
human	WB	Haematologica; Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.; Authors - Stephan Michalik
mouse	WB	Neurobiol Dis; Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models; Authors - Rodrigo Vinueza-Gavilanes

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