Anticorps Monoclonal anti-14-3-3E

14-3-3E Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

3A3A4

N° de cat : 66946-1-PBS

Synonymes

14-3-3 epsilon, YWHAE, 14-3-3 protein epsilon, 14-3-3epsilon

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Galerie de données de validation

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  WB analysis using 66946-1-Ig (same clone as 66946-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66946-1-Ig (14-3-3E antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66946-1-Ig (14-3-3E antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66946-1-PBS cible 14-3-3E dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: 14-3-3E Protéine recombinante Ag25186
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Masse moléculaire calculée: 255 aa, 29 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC000179
• Symbole du gène: 14-3-3E
• Identification du gène (NCBI): 7531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

14-3-3 epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

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