Anticorps Polyclonal de lapin anti-14-3-3 GAMMA-Specific

• Phare
• Validé par KD/KO

14-3-3 GAMMA-Specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 12381-1-AP

Synonymes

14 3 3 protein gamma, 14 3 3GAMMA, 14-3-3, 14-3-3 GAMMA, KCIP 1, YWHAG

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Galerie de données de validation

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  WB analysis using 12381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse IMCD3 cells (RNAi) using 12381-1-AP

  siRNA result of 12381-1-AP (anti-14-3-3G) in Mouse IMCD3 cells From Dr.Seongjin Seo.

• 

  WB analysis of A431 using 12381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12381-1-AP

  IP Result of anti-14-3-3 GAMMA-Specific (IP:12381-1-AP, 3ug; Detection:12381-1-AP 1:800) with mouse brain tissue lysate 8000ug.

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  IHC staining of human lung cancer using 12381-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12381-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 12381-1-AP

  Immunofluorescent analysis of MDCK cells using 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules IMCD3 de souris (ARNi), cellules A431, cellules K-562, cellules NIH/3T3
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

12381-1-AP cible 14-3-3 GAMMA-Specific dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: 14-3-3 GAMMA-Specific Protéine recombinante Ag3047
• Nom complet: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 28-33 kDa
• Numéro d’acquisition GenBank: BC020963
• Symbole du gène: YWHAG
• Identification du gène (NCBI): 7532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

14-3-3 gamma (also known as YWHAG) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody specifically recognizes gamma isoform of 14-3-3.

Publications

Species	Application	Title
human	CoIP,WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
human	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Human adipocyte differentiation and composition of disease-relevant lipids are regulated by miR-221-3p.; Authors - Maria A Ahonen
human	WB	Proteomics; Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells.; Authors - Songlin Zeng
human	WB,IP	J Exp Clin Cancer Res; 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.; Authors - Jia Song
human	CoIP	Autophagy; YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization.; Authors - Yang Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Erik (Verified Customer) (08-26-2021)

We used the antibody at 1:1000 dilution with 11ug input protein, and it produced one clean and strong band at the expected height for the protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MIAPACA2 and PANC1 cell lines

14-3-3 GAMMA-Specific Antibody Western Blot validation (1:1000 dilution) in MIAPACA2 and PANC1 cell lines (Cat no:12381-1-AP)